TY - JOUR T1 - Cellular segregation in co-cultures driven by differential adhesion and contractility on distinct time scales JF - bioRxiv DO - 10.1101/2022.05.23.492966 SP - 2022.05.23.492966 AU - Mark Skamrahl AU - Justus Schünemann AU - Markus Mukenhirn AU - Jannis Gottwald AU - Maximilian Ferle AU - Angela Rübeling AU - Alf Honigmann AU - Andreas Janshoff Y1 - 2022/01/01 UR - http://biorxiv.org/content/early/2022/05/24/2022.05.23.492966.abstract N2 - Cellular sorting and pattern formation is crucial for many biological processes such as development, tissue regeneration, and cancer progression. Prominent physical driving forces for cellular sorting are differential adhesion and contractility. Here, we studied the segregation of epithelial co-cultures containing highly contractile, ZO1/2-depleted MDCKII cells (dKD) and their wildtype (WT) counterparts using multiple quantitative, high-throughput methods to monitor their dynamical and mechanical properties. We observe a time-dependent segregation process, governed mainly by differential contractility on short (< 5 h) and differential adhesion on long (> 5 h) time scales, respectively. The overly contractile dKD cells exert strong lateral forces on their WT neighbors, thereby apically depleting their surface area. This is reflected in a six-fold difference in excess surface area between both cell types. The lateral forces lead to a four- to six-fold increase in tension at all junctions that are in contact with the contractile cells including the interface between heterotypic cell-cell contacts. Concomitantly, the tight junction-depleted, contractile cells exhibit weaker cell-cell adhesion. Drug-induced contractility reduction delays the initial segregation but ceases to change the final demixed state, rendering differential adhesion the dominant segregation force at longer time scales.This well-controlled model system shows how cell sorting is accomplished through a complex interplay between differential adhesion and contractility and can be explained largely by generic physical driving forces.Competing Interest StatementThe authors have declared no competing interest. ER -