RT Journal Article
SR Electronic
T1 Endothelial Brg1 fine-tunes Notch signaling during zebrafish heart regeneration
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.05.18.492445
DO 10.1101/2022.05.18.492445
A1 Chenglu Xiao
A1 Junjie Hou
A1 Fang Wang
A1 Yabing Song
A1 Jiyuan Zheng
A1 Lingfei Luo
A1 Jianbin Wang
A1 Wanqiu Ding
A1 Xiaojun Zhu
A1 Jing-Wei Xiong
YR 2022
UL http://biorxiv.org/content/early/2022/05/25/2022.05.18.492445.abstract
AB Myocardial Brg1 is essential for heart regeneration in zebrafish, but it remains unknown whether and how endothelial Brg1 plays a role in heart regeneration. Here, we found that both brg1 mRNA and protein were induced in cardiac endothelial cells after ventricular resection, and endothelium-specific over-expression of dominant-negative Xenopus Brg1 (DN-xBrg1) inhibited myocardial proliferation and heart regeneration and increased cardiac fibrosis. RNA-seq and ChIP-seq analysis revealed that the endothelium-specific over-expression of DN-xBrg1 changed the levels of H3K4me3 modifications in the promoter regions of the zebrafish genome and induced abnormal activation of Notch family genes upon injury. Mechanistically, Brg1 interacted with lysine demethylase 7aa (Kdm7aa) to fine-tune the level of H3K4me3 within the promoter regions of Notch family genes and thus regulated Notch gene transcription. Together, this work demonstrates that the Brg1-Kdm7aa-Notch axis in cardiac endothelial cells, including the endocardium, regulates myocardial proliferation and regeneration via modulating the H3K4me3 of the Notch promoters in zebrafish.Competing Interest StatementThe authors have declared no competing interest.