TY - JOUR T1 - A super robust and efficient DNA storage architecture based on modulation encoding and decoding JF - bioRxiv DO - 10.1101/2022.05.25.490755 SP - 2022.05.25.490755 AU - Xiangzhen Zan AU - Ranze Xie AU - Xiangyu Yao AU - Peng Xu AU - Wenbin Liu Y1 - 2022/01/01 UR - http://biorxiv.org/content/early/2022/05/25/2022.05.25.490755.abstract N2 - Thanks to its high density and long durability, synthetic DNA has been widely considered as a promising solution to the data explosion problem. However, due to the large amount of random base insertion-deletion-substitution (IDSs) errors from sequencing, reliable data recovery remains a critical challenge, which hinders its large-scale application. Here, we propose a modulation-based DNA storage architecture. Experiments on simulation and real datasets demonstrate that it has two distinct advantages. First, modulation encoding provides a simple way to ensure the encoded DNA sequences comply with biological sequence constraints (i.e., GC balanced and no homopolymers); Second, modulation decoding is highly efficient and extremely robust for the detection of insertions and deletions, which can correct up to ∼40% errors. These two advantages pave the way for future high-throughput and low-cost techniques, and will kickstart the actualization of a viable, large-scale system for DNA data storage.Competing Interest StatementThe authors have declared no competing interest. ER -