RT Journal Article
SR Electronic
T1 CSR-1 RNA interference pathway restricts holocentromere protein CENP-A/HCP-3 localization in <em>Caenorhabditis elegans</em>
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.05.26.493264
DO 10.1101/2022.05.26.493264
A1 Charmaine Yan Yu Wong
A1 Karen Wing Yee Yuen
YR 2022
UL http://biorxiv.org/content/early/2022/05/26/2022.05.26.493264.abstract
AB CSR-1 is an argonaute of a RNA interference pathway that is important for chromosome segregation in C. elegans. Live-cell imaging revealed that CSR-1 depletion slows down spindle pole separation in a kinetochore-dependent manner. In csr-1(RNAi) embryos, the kinetochores may be misattached to the microtubules and chromosome segregation is disrupted. On the holocentromeres, there are increased levels of some kinetochore proteins, including the centromeric epigenetic mark, CENP-A or HCP-3. Without affecting HCP-3 expression level, HCP-3 density is higher on stretched chromatin fibers in CSR-1-depleted embryos. The increased HCP-3 deposition on chromatin after CSR-1 depletion is at least partially independent of HCP-3 loading factors, KNL-2 and LIN-53, suggesting a non-classical, improper HCP-3 loading pathway. Negative regulation of HCP-3 holocentromere loading by CSR-1 required its slicer activity and the b isoform. CSR-1 acts as a HCP-3 repressor for its chromosomal occupancy, shedding light on the role of RNAi pathways in specifying the localization of centromere proteins.Competing Interest StatementThe authors have declared no competing interest.