PT  - JOURNAL ARTICLE
AU  - Ravi D. Nath
AU  - Claire N. Bedbrook
AU  - Rahul Nagvekar
AU  - Karl Deisseroth
AU  - Anne Brunet
TI  - Rapid and precise genome engineering in a naturally short-lived vertebrate
AID  - 10.1101/2022.05.25.493454
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.05.25.493454
4099  - http://biorxiv.org/content/early/2022/05/26/2022.05.25.493454.short
4100  - http://biorxiv.org/content/early/2022/05/26/2022.05.25.493454.full
AB  - The African turquoise killifish is a powerful vertebrate system to study complex phenotypes at scale, including aging and age-related disease. Here we develop a rapid and precise CRISPR/Cas9-mediated knock-in approach in the killifish. We show its efficient application to precisely insert fluorescent reporters of different sizes at various genomic loci, to drive cell-type- and tissue-specific expression. This knock-in method should allow the establishment of humanized disease models and the development of cell-type-specific molecular probes for studying complex vertebrate biology.Competing Interest StatementThe authors have declared no competing interest.CNcortical nucleusCPcentral posterior thalamic nucleusCpostposterior commissureDILdiffuse inferior lobe of hypothalamusglglomerular layerHahabenular nucleusHccaudal hypothalamusHddorsal hypothalamusHvventral hypothalamusllflateral longitudinal fascicleLRlateral recess of diencephalic ventriclemlfmedial longitudinal fascicleMOmedulla oblongataNGglomerular nucleusOBolfactory bulbONoptic nerveOToptic tectumPGZperiglomerular gray zoneTeltelencephalonTltorus longitudinalisTNaanterior tuberal nucleusTPpperiventricular nucleus of posterior tuberculumVavalvula of cerebellumVAOventral accessory optic nucleus