RT Journal Article
SR Electronic
T1 Rapid and precise genome engineering in a naturally short-lived vertebrate
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.05.25.493454
DO 10.1101/2022.05.25.493454
A1 Ravi D. Nath
A1 Claire N. Bedbrook
A1 Rahul Nagvekar
A1 Karl Deisseroth
A1 Anne Brunet
YR 2022
UL http://biorxiv.org/content/early/2022/05/26/2022.05.25.493454.abstract
AB The African turquoise killifish is a powerful vertebrate system to study complex phenotypes at scale, including aging and age-related disease. Here we develop a rapid and precise CRISPR/Cas9-mediated knock-in approach in the killifish. We show its efficient application to precisely insert fluorescent reporters of different sizes at various genomic loci, to drive cell-type- and tissue-specific expression. This knock-in method should allow the establishment of humanized disease models and the development of cell-type-specific molecular probes for studying complex vertebrate biology.Competing Interest StatementThe authors have declared no competing interest.CNcortical nucleusCPcentral posterior thalamic nucleusCpostposterior commissureDILdiffuse inferior lobe of hypothalamusglglomerular layerHahabenular nucleusHccaudal hypothalamusHddorsal hypothalamusHvventral hypothalamusllflateral longitudinal fascicleLRlateral recess of diencephalic ventriclemlfmedial longitudinal fascicleMOmedulla oblongataNGglomerular nucleusOBolfactory bulbONoptic nerveOToptic tectumPGZperiglomerular gray zoneTeltelencephalonTltorus longitudinalisTNaanterior tuberal nucleusTPpperiventricular nucleus of posterior tuberculumVavalvula of cerebellumVAOventral accessory optic nucleus