TY - JOUR T1 - Unbiased stereological estimates of dopaminergic and GABAergic neurons in the A10, A9, and A8 subregions in the young male Macaque JF - bioRxiv DO - 10.1101/2022.02.08.479596 SP - 2022.02.08.479596 AU - Emily A. Kelly AU - Jancy Contreras AU - Annie Duan AU - Rochelle Vassell AU - Julie L. Fudge Y1 - 2022/01/01 UR - http://biorxiv.org/content/early/2022/05/26/2022.02.08.479596.abstract N2 - The ventral midbrain is the primary source of dopamine- (DA) expressing neurons in most species. GABA-ergic and glutamatergic cell populations are intermixed among DA-expressing cells and purported to regulate both local and long-range dopamine neuron activity. Most work has been conducted in rodent models, however due to evolutionary expansion of the ventral midbrain in primates, the increased size and complexity of DA subpopulations warrants further investigation. Here, we quantified the number of DA neurons, and their GABA-ergic complement in classic DA cell groups A10 (midline ventral tegmental area nuclei [VTA] and parabrachial pigmented nucleus [PBP]), A9 (substantia nigra, pars compacta [SNc]) and A8 (retrorubral field [RRF]) in the macaque. Because the PBP is a disproportionately expanded feature of the A10 group, and has unique connectional features in monkeys, we analyzed A10 data by dividing it into ‘classic’ midline nuclei and the PBP. Unbiased stereology revealed total putative DA neuron counts to be 210,238 +/− 17,127 (A10 = 110,319 +/− 9,649, A9= 87,399 +/−7,751 and A8=12,520 +/− 827). Putative GABAergic neurons were fewer overall, and evenly dispersed across the DA subpopulations (GAD67= 71,215 +/− 5,663; A10=16,836 +/− 2,743; A9=24,855 +/− 3,144 and A8=12,633 +/− 3,557). Calculating the GAD67/TH ratio for each subregion revealed differential balances of these two cell types across the DA subregions. The A8 subregion had the highest complement of GAD67-positive neurons compared to TH-positive neurons (1:1), suggesting a potentially high capacity for GABAergic inhibition of DA output in this region.HIGHLIGHTSThe A10 subregion expands laterally and caudally in nonhuman primatesThe A10, A9, and A8 comprise 52%, 42% and 6% of DA neurons, respectivelyGABAergic neurons are more evenly dispersed across subregionsThe A8 subpopulation has the highest ratio of GABA: DA neuronsCompeting Interest StatementThe authors have declared no competing interest.CaBPcalcium binding protein D28kCLicaudal linear nucleusDAdopamineIIIthird nerve rootGABAGamma-Aminobutyric AcidGAD67glutamic acid decarboxylase 67GluGlutamateIFintrafascicular nucleusIPinterpeduncular nucleusPBPparabrachial pigmented nucleusPNparanigral nucleusRNred nucleusRRFretrorubral fieldRMTgrostromedial tegmental nucleusscpdecussation of the superior cerebellar peduncleSNsubstantia nigraSNcsubstantia nigra, pars compactaSNrsubstantia nigra, pars reticulataSTNsubthalamic nucleusTHtyrosine hydroxylaseVTAventral tegmental area. ER -