PT  - JOURNAL ARTICLE
AU  - Gilad Almogy
AU  - Mark Pratt
AU  - Florian Oberstrass
AU  - Linda Lee
AU  - Dan Mazur
AU  - Nate Beckett
AU  - Omer Barad
AU  - Ilya Soifer
AU  - Eddie Perelman
AU  - Yoav Etzioni
AU  - Martin Sosa
AU  - April Jung
AU  - Tyson Clark
AU  - Eliane Trepagnier
AU  - Gila Lithwick-Yanai
AU  - Sarah Pollock
AU  - Gil Hornung
AU  - Maya Levy
AU  - Matthew Coole
AU  - Tom Howd
AU  - Megan Shand
AU  - Yossi Farjoun
AU  - James Emery
AU  - Giles Hall
AU  - Samuel Lee
AU  - Takuto Sato
AU  - Ricky Magner
AU  - Sophie Low
AU  - Andrew Bernier
AU  - Bharathi Gandi
AU  - Jack Stohlman
AU  - Corey Nolet
AU  - Siobhan Donovan
AU  - Brendan Blumenstiel
AU  - Michelle Cipicchio
AU  - Sheila Dodge
AU  - Eric Banks
AU  - Niall Lennon
AU  - Stacey Gabriel
AU  - Doron Lipson
TI  - Cost-efficient whole genome-sequencing using novel mostly natural sequencing-by-synthesis chemistry and open fluidics platform
AID  - 10.1101/2022.05.29.493900
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.05.29.493900
4099  - http://biorxiv.org/content/early/2022/06/03/2022.05.29.493900.short
4100  - http://biorxiv.org/content/early/2022/06/03/2022.05.29.493900.full
AB  - We introduce a massively parallel novel sequencing platform that combines an open flow cell design on a circular wafer with a large surface area and mostly natural nucleotides that allow optical end-point detection without reversible terminators. This platform enables sequencing billions of reads with longer read length (∼300bp) and fast runs times (<20hrs) with high base accuracy (Q30 > 85%), at a low cost of $1/Gb. We establish system performance by whole-genome sequencing of the Genome-In-A-Bottle reference samples HG001-7, demonstrating high accuracy for SNPs (99.6%) and Indels in homopolymers up to length 10 (96.4%) across the vast majority (>98%) of the defined high-confidence regions of these samples. We demonstrate scalability of the whole-genome sequencing workflow by sequencing an additional 224 selected samples from the 1000 Genomes project achieving high concordance with reference data.Competing Interest StatementGA, MP, FO, LL, DM, NB, OB, IS, EP, YE, MS, AJ, TC, GL-Y, SP, GH, ML and DL are employees and share holders of Ultima Genomics