RT Journal Article
SR Electronic
T1 Transcriptional activity is shaped by the chromatin landscapes in Arabidopsis
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.06.02.494419
DO 10.1101/2022.06.02.494419
A1 Bhagyshree Jamge
A1 Zdravko J. Lorković
A1 Elin Axelsson
A1 Ramesh Yelagandula
A1 Svetlana Akimcheva
A1 Frédéric Berger
YR 2022
UL http://biorxiv.org/content/early/2022/06/03/2022.06.02.494419.abstract
AB How histone variants and histone modifications shape nucleosome-mediated transcriptional repression, and how transcriptional activity shapes the enrichment of histone modifications and variants remain unclear. To clarify these relationships, we examined chromatin organization in the Arabidopsis thaliana genome, identifying a limited number of chromatin landscapes that distinguish transposon families and distinct groups of genes based on their transcriptional regulation. Unexpectedly, H2A variants are strong determinants of the landscape architecture. Combinations of H2A.W and four histone modifications define six domains that are occupied by specific transposon families and organized concentrically around the centromere. Moreover, H2A.Z defines transcriptional gene repression in specific domains. Expressed genes occupy four chromatin landscapes with specific RNA Polymerase II profiles. Although the composition of each chromatin landscape is invariant, they cover genes with a wide range of expression levels. Therefore, chromatin landscapes control the range of transcriptional activity, but transcriptional activity has little effect on chromatin composition.One Sentence Summary Histone variants and histone modifications build a limited number of distinct chromatin landscapes that instruct the transcriptional regulation of genes and transposons in Arabidopsis.Competing Interest StatementThe authors have declared no competing interest.