PT  - JOURNAL ARTICLE
AU  - Alshafie, Walaa
AU  - Fotouhi, Maryam
AU  - Shlaifer, Irina
AU  - Edwards, Aled M.
AU  - Durcan, Thomas M
AU  - McPherson, Peter S.
AU  - Laflamme, Carl
TI  - The identification of potent and selective antibodies for Serine/threonine-protein kinase TBK1, for use in immunoblot, immunofluorescence and immunoprecipitation
AID  - 10.1101/2022.06.03.494699
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.06.03.494699
4099  - http://biorxiv.org/content/early/2022/06/03/2022.06.03.494699.short
4100  - http://biorxiv.org/content/early/2022/06/03/2022.06.03.494699.full
AB  - TBK1 is a serine-threonine kinase that has been linked to a number of diseases, including amyotrophic lateral sclerosis and frontotemporal dementia. Reproducible research on TBK1 has been hampered by the lack of well characterized antibodies. In this study, we characterized 11 commercial antibodies for immunoblot, immunofluorescence and immunoprecipitation, using a knock-out cell line as the control. For each application, we identified several potent and selective antibodies that will facilitate studies on TBK1.Competing Interest StatementThe authors have declared no competing interest.