RT Journal Article
SR Electronic
T1 The identification of potent and selective antibodies for Serine/threonine-protein kinase TBK1, for use in immunoblot, immunofluorescence and immunoprecipitation
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.06.03.494699
DO 10.1101/2022.06.03.494699
A1 Alshafie, Walaa
A1 Fotouhi, Maryam
A1 Shlaifer, Irina
A1 Edwards, Aled M.
A1 Durcan, Thomas M
A1 McPherson, Peter S.
A1 Laflamme, Carl
YR 2022
UL http://biorxiv.org/content/early/2022/06/03/2022.06.03.494699.abstract
AB TBK1 is a serine-threonine kinase that has been linked to a number of diseases, including amyotrophic lateral sclerosis and frontotemporal dementia. Reproducible research on TBK1 has been hampered by the lack of well characterized antibodies. In this study, we characterized 11 commercial antibodies for immunoblot, immunofluorescence and immunoprecipitation, using a knock-out cell line as the control. For each application, we identified several potent and selective antibodies that will facilitate studies on TBK1.Competing Interest StatementThe authors have declared no competing interest.