PT  - JOURNAL ARTICLE
AU  - Daniëlle de Jong-Bolm
AU  - Mohsen Sadeghi
AU  - Guobin Bao
AU  - Gabriele Klaehn
AU  - Merle Hoff
AU  - Lucas Mittelmeier
AU  - F. Buket Basmanav
AU  - Felipe Opazo
AU  - Frank Noé
AU  - Silvio O. Rizzoli
TI  - Protein nanobarcodes enable single-step multiplexed fluorescence imaging
AID  - 10.1101/2022.06.03.494744
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.06.03.494744
4099  - http://biorxiv.org/content/early/2022/06/04/2022.06.03.494744.short
4100  - http://biorxiv.org/content/early/2022/06/04/2022.06.03.494744.full
AB  - Multiplexed cellular imaging typically relies on the sequential application of detection probes, such as antibodies or DNA barcodes, which is complex and time-consuming. To address this, we developed here protein nanobarcodes, composed of combinations of epitopes recognized by specific sets of nanobodies. The nanobarcodes are read in a single imaging step, relying on nanobodies conjugated to distinct fluorophores, which enables a precise analysis of large numbers of protein combinations.Competing Interest StatementS.O.R. and F.O. are shareholders of NanoTag Biotechnologies GmbH. All other authors declare no potential conflict of interest.