PT  - JOURNAL ARTICLE
AU  - Andrea J. Radtke
AU  - Ekaterina Postovalova
AU  - Arina Varlamova
AU  - Alexander Bagaev
AU  - Maria Sorokina
AU  - Olga Kudryashova
AU  - Mark Meerson
AU  - Margarita Polyakova
AU  - Ilia Galkin
AU  - Viktor Svekolkin
AU  - Sergey Isaev
AU  - Grigory Perelman
AU  - Yaroslav Lozinsky
AU  - Ziv Yaniv
AU  - Bradley C. Lowekamp
AU  - Emily Speranza
AU  - Li Yao
AU  - Stefania Pittaluga
AU  - Arthur L. Shaffer III
AU  - Danny Jonigk
AU  - James D. Phelan
AU  - Theresa Davies-Hill
AU  - Da Wei Huang
AU  - Pavel Ovcharov
AU  - Krystle Nomie
AU  - Ekaterina Nuzhdina
AU  - Nikita Kotlov
AU  - Ravshan Ataullakhanov
AU  - Nathan Fowler
AU  - Michael Kelly
AU  - Jagan Muppidi
AU  - Jeremy Davis
AU  - Jonathan M. Hernandez
AU  - Wyndham H. Wilson
AU  - Elaine S. Jaffe
AU  - Louis M. Staudt
AU  - Mark Roschewski
AU  - Ronald N. Germain
TI  - A Multi-scale, Multiomic Atlas of Human Normal and Follicular Lymphoma Lymph Nodes
AID  - 10.1101/2022.06.03.494716
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.06.03.494716
4099  - http://biorxiv.org/content/early/2022/06/05/2022.06.03.494716.short
4100  - http://biorxiv.org/content/early/2022/06/05/2022.06.03.494716.full
AB  - Reference atlases, molecular and spatial maps of mammalian tissues, are critical resources for discovery efforts and translational research. Their utility is dependent on operationalizing the resulting data by identifying cell types, histological patterns, and predictive biomarkers underlying health and disease. The human lymph node (LN) offers a compelling use case because of its importance in immunity, structural and cellular diversity, and neoplastic involvement. One hematological malignancy, follicular lymphoma (FL), evolves from developmentally blocked germinal center B cells residing in and trafficking through these tissues. To promote survival and immune escape, tumor B cells undergo significant genetic changes and extensively remodel the lymphoid microenvironment. Here, we present an integrated portrait of healthy and FL LNs using multiple genomic and advanced imaging technologies. By leveraging the strengths of each platform, we identified several tumor-specific features and microenvironmental patterns enriched in individuals who experience early relapse, the most high-risk of FL patients.Competing Interest StatementNathan Fowler is the Chief Medical Officer of BostonGene, Corp and a Professor at the University of Texas MD Anderson Cancer Center. Ekaterina Postovalova, Arina Varlamova, Alexander Bagaev, Maria Sorokina, Olga Kudryashova, Alexander Bagaev, Mark Meerson, Margarita Polyakova, Ilia Galkin, Viktor Svekolkin, Sergei Isaev, Grigory Perelman, Yaroslav Lozinsky, Pavel Ovcharov, Krystle Nomie, Ekaterina Nuzhdina, Nikita Kotlov, Ravshan Attaulakhanov were employees at BostonGene at the time the study was performed. Ekaterina Postovalova, Arina Varlamova, Alexander Bagaev, Olga Kudryashova, Mark Meerson, Ilia Galkin, Viktor Svekolkin, Pavel Ovcharov, Ekaterina Nuzhdina, Nikita Kotlov, Ravshan Attaulakhanov are inventors on patent applications related to MxIF pipelines and proprietary BostonGene software. All other authors declare no other competing financial interests.