RT Journal Article
SR Electronic
T1 Melittin-induced alterations in morphology and deformability of human red blood cells using quantitative phase imaging techniques
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 091991
DO 10.1101/091991
A1 Joonseok Hur
A1 Kyoohyun Kim
A1 SangYun Lee
A1 HyunJoo Park
A1 YongKeun Park
YR 2016
UL http://biorxiv.org/content/early/2016/12/06/091991.abstract
AB Here, the actions of melittin, the active molecule of apitoxin or bee venom, were investigated on human red blood cells (RBCs) using quantitative phase imaging techniques. High-resolution realtime 3-D refractive index (RI) measurements and dynamic 2-D phase images of individual melittin-bound RBCs enabled in-depth examination of melittin-induced biophysical alterations of the cells. From the measurements, morphological, biochemical, and mechanical alterations of the RBCs were analyzed quantitatively. Furthermore, leakage of haemoglobin (Hb) inside the RBCs at high melittin concentration was also investigated.