RT Journal Article
SR Electronic
T1 Glutamine metabolism modulates chondrocyte inflammatory response
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.06.09.495504
DO 10.1101/2022.06.09.495504
A1 Arra, Manoj
A1 Swarnkar, Gaurav
A1 Adapala, Naga Suresh
A1 Naqvi, Syeda Kanwal
A1 Cai, Lei
A1 Rai, Farooq
A1 Mbalaviele, Gabriel
A1 Brophy, Robert
A1 Abu-Amer, Yousef
YR 2022
UL http://biorxiv.org/content/early/2022/06/09/2022.06.09.495504.abstract
AB Osteoarthritis is the most common joint disease in the world with significant societal consequences, but lacks effective disease modifying interventions. The pathophysiology consists of a prominent inflammatory component that can be targeted to prevent cartilage degradation and structural defects. Intracellular metabolism has emerged as a culprit of the inflammatory response in chondrocytes, with both processes co-regulating each other. The role of glutamine metabolism in chondrocytes, especially in the context of inflammation, lacks a thorough understanding and is the focus of this work. We display that chondrocytes utilize glutamine for energy production and anabolic processes. Furthermore, we show that glutamine deprivation itself causes metabolic reprogramming and decreases the inflammatory response of chondrocytes through inhibition of NF-κB activity. Finally, we display that glutamine deprivation promotes autophagy and that ammonia is an inhibitor of autophagy. Overall, we identify a relationship between glutamine metabolism and inflammatory signaling and display the need for increased study of chondrocyte metabolic systems.Competing Interest StatementThe authors have declared no competing interest.