RT Journal Article
SR Electronic
T1 Increasing the Field-of-View in Oblique Plane Microscopy via optical tiling
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.06.12.495844
DO 10.1101/2022.06.12.495844
A1 Chen, Bingying
A1 Chang, Bo-Jui
A1 Zhou, Felix
A1 Daetwyler, Stephan
A1 Sapoznik, Etai
A1 Gihana, Gabriel M.
A1 Castro, Lizbeth Perez
A1 Sorrell, Maralice Conacci
A1 Dean, Kevin M.
A1 Millett-Sikking, Alfred
A1 York, Andrew G.
A1 Fiolka, Reto
YR 2022
UL http://biorxiv.org/content/early/2022/06/13/2022.06.12.495844.abstract
AB Fast volumetric imaging of large fluorescent samples with high-resolution is required for many biological applications. Oblique plane microscopy (OPM) provides high spatiotemporal resolution, but the field of view is typically limited by its optical train and the pixel number of the camera. Mechanically scanning the sample or decreasing the overall magnification of the imaging system can partially address this challenge, albeit by reducing the volumetric imaging speed or spatial sampling, respectively. In this Letter, we introduce a novel dual-axis scan unit for OPM that enables rapid and high-resolution volumetric imaging throughout a volume of 800 × 500 × 200 microns. This enables imaging of model organisms, such as zebrafish embryos, with subcellular resolution. Furthermore, we combined this microscope with a real-time and multi-perspective projection imaging technique to increase the volumetric interrogation rate to more than 10 Hz.Competing Interest StatementThe authors have declared no competing interest.