RT Journal Article
SR Electronic
T1 A quantitative gibberellin signalling biosensor reveals a role for gibberellins in internode specification at the shoot apical meristem
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.06.11.448154
DO 10.1101/2021.06.11.448154
A1 Bihai Shi
A1 Amelia Felipo-Benavent
A1 Guillaume Cerutti
A1 Carlos Galvan-Ampudia
A1 Lucas Jilli
A1 Geraldine Brunoud
A1 Jérome Mutterer
A1 Elody Vallet
A1 Lali Sakvarelidze-Achard
A1 Jean-Michel Davière
A1 Alejandro Navarro-Galiano
A1 Ankit Walia
A1 Shani Lazary
A1 Jonathan Legrand
A1 Roy Weinstain
A1 Alexander M. Jones
A1 Salomé Prat
A1 Patrick Achard
A1 Teva Vernoux
YR 2022
UL http://biorxiv.org/content/early/2022/06/15/2021.06.11.448154.abstract
AB Growth at the shoot apical meristem (SAM) is essential for shoot architecture construction. The phytohormones gibberellins (GA) play a pivotal role in coordinating plant growth, but their role in the SAM remains mostly unknown. Here, we developed a ratiometric GA signalling biosensor by engineering one of the DELLA proteins, to suppress its master regulatory function in GA transcriptional responses while preserving its degradation upon GA sensing. We demonstrate that this novel degradation-based biosensor accurately reports on cellular changes in GA levels and perception during development. We used this biosensor to map GA signalling activity in the SAM. We show that high GA signalling is found primarily in cells located between organ primordia that are the precursors of internodes. By gain- and loss-of-function approaches, we further demonstrate that GAs regulate cell division plane orientation to establish the typical cellular organisation of internodes, thus contributing to internode specification in the SAM.Competing Interest StatementThe authors have declared no competing interest.