PT  - JOURNAL ARTICLE
AU  - Myers-Turnbull, Douglas
AU  - Taylor, Jack C
AU  - Helsell, Cole
AU  - McCarroll, Matthew N
AU  - Ki, Chris S
AU  - Tummino, Tia A
AU  - Ravikumar, Shreya
AU  - Kinser, Reid
AU  - Gendelev, Leo
AU  - Alexander, Rebekah
AU  - Keiser, Michael J
AU  - Kokel, David
TI  - Simultaneous analysis of neuroactive compounds in zebrafish
AID  - 10.1101/2020.01.01.891432
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2020.01.01.891432
4099  - http://biorxiv.org/content/early/2022/06/15/2020.01.01.891432.short
4100  - http://biorxiv.org/content/early/2022/06/15/2020.01.01.891432.full
AB  - Neuroactive compounds are crucial tools in drug discovery and neuroscience, but it remains difficult to discover neuroactive compounds with new mechanisms of action. To address this need, researchers have developed mid-throughput phenotype-first approaches using zebrafish. This study introduces an open, non-commercial, and extensible hardware/software platform that captures and analyzes drugmodulated phenotypic responses larval zebrafish. We provide full specifications, computer-aided design (CAD) documents, and source code. Accompanying this study, we are also publicly depositing phenotypic data on 3.9 million animals and 34,000 compounds. The data include a high-replicate benchmark set on 14 compounds, a wellcontrolled reference set of 648 known neuroactive compounds, 20 specialized reference sets, a library of 1,520 FDA-approved drugs, 3 screening libraries. This open data resource is curated, structured, tied to extensive metadata, and available under a Creative Commons CC-BY license.Competing Interest StatementThe authors have declared no competing interest.