RT Journal Article
SR Electronic
T1 Porcine ANTXR1, Heparan Sulfate and Neu5Gc act as entry factors for Seneca Valley virus invasion
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.06.14.496051
DO 10.1101/2022.06.14.496051
A1 Wenda Tang
A1 Yanchao Wang
A1 Xiaolan Qi
A1 Fengxing Gu
A1 Kangli Li
A1 Haitang Han
A1 Xuguang Du
A1 Zixiang Zhu
A1 Sen Wu
A1 Yaofeng Zhao
A1 Haixue Zheng
YR 2022
UL http://biorxiv.org/content/early/2022/06/16/2022.06.14.496051.abstract
AB Seneca Valley virus (SVV) disease is a newly emerging infectious disease of pigs caused by SVV, which seriously endangers the pig industry. This study was set out to identify the essential host factors required for SVV entering porcine cells. Using a CRISPR/Cas9 library containing 93,859 sgRNAs that were designed to target approximately 22,707 porcine genes, we generated mutated porcine cell libraries, which were subjected to SVV challenge for enrichment of cells resistant to SVV infection. These resistant cells were subsequently analyzed to identify genes essential for SVV infection. We demonstrated that ANTXR1, a type I transmembrane protein encoded by ANTXR1, heparan sulfate (HS), glycosaminoglycans modified by acetylation and sulfation of HS2ST1, and Neu5Gc, a non-human sialic acid catalyzed by CMAH, were the essential host factors for SVV entry into porcine cells. These results will be helpful to elucidate the pathogenesis of SVV and the development of prevention and control measures.Competing Interest StatementThe authors have declared no competing interest.