PT  - JOURNAL ARTICLE
AU  - Michael Schmitz
AU  - Irma Querques
AU  - Seraina Oberli
AU  - Christelle Chanez
AU  - Martin Jinek
TI  - Structural basis for RNA-mediated assembly of type V CRISPR-associated transposons
AID  - 10.1101/2022.06.17.496590
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.06.17.496590
4099  - http://biorxiv.org/content/early/2022/06/17/2022.06.17.496590.short
4100  - http://biorxiv.org/content/early/2022/06/17/2022.06.17.496590.full
AB  - CRISPR systems have been co-opted by Tn7-like elements to direct RNA-guided transposition. Type V-K CRISPR-associated transposons rely on the concerted activities of the pseudonuclease Cas12k, the AAA+ ATPase TnsC, the Zn-finger protein TniQ, and the transposase TnsB. Here we present a cryo-electron microscopic structure of a target DNA-bound Cas12k-transposon recruitment complex comprising RNA-guided Cas12k, TniQ, TnsC and, unexpectedly, the ribosomal protein S15. Complex assembly on target DNA results in complete R-loop formation mediated by critical interactions between TniQ and the trans-activating crRNA, and is coupled with TniQ-dependent nucleation of a TnsC filament. In vivo transposition assays corroborate our structural findings, and biochemical and functional analyses of S15 supports its role as a bona fide component of the type V crRNA-guided transposition machinery. Altogether, our work uncovers key aspects of the mechanisms underpinning RNA-mediated assembly of CRISPR-associated transposons that will guide their development as programmable site-specific gene insertion tools.Competing Interest StatementThe authors have filed a related patent application.