RT Journal Article
SR Electronic
T1 Three-dimensional mitochondrial fission, fusion and depolarisation event location prediction for a high throughput analysis of fluorescence microscopy images
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.06.27.497752
DO 10.1101/2022.06.27.497752
A1 James Garrett de Villiers
A1 Rensu Petrus Theart
YR 2022
UL http://biorxiv.org/content/early/2022/06/27/2022.06.27.497752.abstract
AB This paper documents the development of a novel method to predict the occurrence and exact locations of mitochondrial fission, fusion and depolarisation events in three dimensions. These occurrence and location of these events were successfully predicted with a three-dimensional version of the Pix2Pix generative adversarial network (GAN) as well as a three-dimensional adversarial segmentation network called the Vox2Vox GAN. The Pix2Pix GAN predicted the locations of mitochondrial fission, fusion and depolarisation events with accuracies of 35.9%, 33.2% and 4.90%, respectively. Similarly, the Vox2Vox GAN achieved accuracies of 37.1%, 37.3% and 7.43%. The accuracies achieved by the networks in this paper are too low for the immediate implementation of these tools in life science research. They do however indicate that the networks have modelled the mitochondrial dynamics to some degree of accuracy and may therefore still be helpful as an indication of where events might occur if time lapse sequences are not available. The prediction of these morphological mitochondrial events have, to our knowledge, never been achieved before in literature. The results from this paper can be used as a baseline for the results obtained by future work.Competing Interest StatementThe authors have declared no competing interest.