RT Journal Article SR Electronic T1 Hspa13 Regulates Endoplasmic Reticulum and Cytosolic Proteostasis Through Modulation of Protein Translocation JF bioRxiv FD Cold Spring Harbor Laboratory SP 2022.06.27.497790 DO 10.1101/2022.06.27.497790 A1 Espinoza, Mateo F. A1 Nguyen, Khanh K. A1 Sycks, Melody M. A1 Lyu, Ziqi A1 Montoya, Maureen R. A1 Genereux, Joseph C. YR 2022 UL http://biorxiv.org/content/early/2022/06/27/2022.06.27.497790.abstract AB Most eukaryotic secretory proteins are co-translationally translocated through Sec61 into the endoplasmic reticulum (ER). Because these proteins have evolved to fold in the ER, their mistargeting is associated with toxicity. Genetic experiments have implicated the ER Hsp70 Hspa13/STCH as involved in processing of nascent secretory proteins. Herein, we evaluate the role of Hspa13 in protein import and the maintenance of cellular proteostasis. We find that Hspa13 interacts primarily with the Sec61 translocon and its associated factors. Hspa13 overexpression inhibits translocation of the secreted protein transthyretin (TTR), leading to accumulation and aggregation of immature TTR in the cytosol. ATPase inactive mutants of Hspa13 further inhibit translocation and maturation of secretory proteins. While Hspa13 overexpression inhibits cell growth and ER quality control, HSPA13 knockout destabilizes proteostasis and increases sensitivity to ER disruption. Thus, we propose that Hspa13 regulates import through the translocon to maintain both ER and cytosolic protein homeostasis.The raw mass spectrometry data associated with this manuscript has been deposited in the PRIDE archive and can be accessed at PXD033498.Competing Interest StatementThe authors have declared no competing interest.