PT  - JOURNAL ARTICLE
AU  - Nathaniel T. Marshall
AU  - Daniel E. Symonds
AU  - Faith M Walker
AU  - Daniel E Sanchez
AU  - Zachary L. Couch
AU  - James D. Kiser, Jr.
TI  - Detecting bat environmental DNA from water-filled road-ruts in upland forest
AID  - 10.1101/2022.06.26.497664
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.06.26.497664
4099  - http://biorxiv.org/content/early/2022/06/29/2022.06.26.497664.short
4100  - http://biorxiv.org/content/early/2022/06/29/2022.06.26.497664.full
AB  - Declines in population and diversity of North American bats are rapidly occurring due to habitat loss, incidental take from various industry projects, and lethal White-nose Syndrome disease. It is critical to accurately census habitat for appropriate conservation measures, yet traditional sampling methodology, such as mist netting and acoustic recordings, can be time-intensive and biased. Instead, a passive sampling tool that does not rely on the a priori knowledge of bat roosts may provide crucial information on bat communities. In the water-limited habitats of forested uplands of the Appalachian Plateau, water-filled road-ruts are important resources for bats. Therefore, we developed an environmental DNA (eDNA) protocol to sample isolated road-ruts that may have the presence of sloughed cellular material from actively drinking bats. The detection of bat eDNA was investigated from a positive control experiment, and across 47 water samples collected in Kentucky and Ohio. Water samples were analyzed using both species-specific quantitative polymerase chain reaction (qPCR) and community metabarcoding methodologies. Using qPCR analysis, we detected eDNA from big brown bat (Eptesicus fuscus) and eastern red bat (Lasiurus borealis) from water-filled road-ruts. While the community metabarcoding approach failed to detect any bat eDNA, many non-target amphibians, birds, and mammals were identified. These results suggest eDNA found within road-ruts provides an additional detection tool for surveying biodiversity across upland forests. Additionally, the use of qPCR increased the detection of rare eDNA targets, which will be crucial for properly implementing future eDNA applications for improving bat conservation efforts across the landscape.Article impact statement Environmental DNA provides detection of bats from drinking sources offering a novel survey method for management and conservation effortsCompeting Interest StatementThe authors have declared no competing interest.