RT Journal Article
SR Electronic
T1 Integrated single-nucleus and spatial transcriptomics captures transitional states in soybean nodule symbiosis establishment
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.06.30.498286
DO 10.1101/2022.06.30.498286
A1 Zhijian Liu
A1 Xiangying Kong
A1 Yanping Long
A1 Hong Zhang
A1 Jinbu Jia
A1 Lijuan Qiu
A1 Jixian Zhai
A1 Zhe Yan
YR 2022
UL http://biorxiv.org/content/early/2022/07/02/2022.06.30.498286.abstract
AB Legumes form symbiosis with rhizobium leading to the development of nitrogen-fixing nodules. By integrating single-nucleus and spatial transcriptomics, we established a cell atlas of soybean nodules and roots. In central infected zone of nodule, we found that uninfected cells specialize into functionally distinct sub-groups during nodule development and revealed a transitional subtype of infected cells with enriched nodulation-related genes. Overall, our results provide a single-cell perspective for understanding rhizobium-legume symbiosis.Competing Interest StatementThe authors have declared no competing interest.