PT  - JOURNAL ARTICLE
AU  - Dinesh Sundaravadivelu Devarajan
AU  - Shiv Rekhi
AU  - Arash Nikoubashman
AU  - Young C. Kim
AU  - Michael P. Howard
AU  - Jeetain Mittal
TI  - Effect of Charge Distribution on the Dynamics of Polyampholytic Disordered Proteins
AID  - 10.1101/2022.07.04.498718
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.07.04.498718
4099  - http://biorxiv.org/content/early/2022/07/04/2022.07.04.498718.short
4100  - http://biorxiv.org/content/early/2022/07/04/2022.07.04.498718.full
AB  - The stability and physiological function of many biomolecular coacervates depend on the structure and dynamics of intrinsically disordered proteins (IDPs) that typically contain a significant fraction of charged residues. Although the effect of relative arrangement of charged residues on IDP conformation is a well-studied problem, the associated changes in dynamics are far less understood. In this work, we systematically interrogate the effects of charge distribution on the chain-level and segmental dynamics of polyampholytic IDPs in dilute solutions. We study a coarse-grained model polyampholyte consisting of an equal fraction of two oppositely charged residues (glutamic acid and lysine) that undergoes a transition from an ideal chain-like conformation for uniformly charge-patterned sequences to a semi-compact conformation for highly charge-segregated sequences. Changes in the chain-level dynamics with increasing charge segregation correlate with changes in conformation. The chain-level and segmental dynamics conform to simple homopolymer models for uniformly charge-patterned sequences but deviate with increasing charge segregation, both in the presence and absence of hydrodynamic interactions. We discuss the significance of these findings, obtained for a model polyampholyte, in the context of a charge-rich intrinsically disordered region of the naturally occurring protein LAF-1. Our findings have important implications for understanding the effects of charge patterning on the dynamics of polyampholytic IDPs in dilute conditions using polymer scaling theories.Competing Interest StatementThe authors have declared no competing interest.