RT Journal Article
SR Electronic
T1 Mapping the Spatial Dynamics of the Human Oral Mucosa in Chronic Inflammatory Disease
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.07.05.498778
DO 10.1101/2022.07.05.498778
A1 Ana J. Caetano
A1 Yushi Redhead
A1 Farah Karim
A1 Pawan Dhami
A1 Shichina Kannambath
A1 Rosamond Nuamah
A1 Ana A. Volponi
A1 Luigi Nibali
A1 Veronica Booth
A1 Eleanor M. D’Agostino
A1 Paul T. Sharpe
YR 2022
UL http://biorxiv.org/content/early/2022/07/05/2022.07.05.498778.abstract
AB The interplay among different cells in a tissue is essential for maintaining homeostasis. Although, disease states have been traditionally attributed to individual cell types, increasing evidence and new therapeutic options have demonstrated the primary role of multicellular functions to understand health and disease, opening new avenues to understand pathogenesis and develop new treatment strategies. We recently described the cellular composition and dynamics of the human oral mucosa; however, the spatial arrangement of cells is needed to better understand a morphologically complex tissue. Here, we link single-cell RNA sequencing, spatial transcriptomics, and high-resolution multiplex fluorescence in situ hybridisation to characterise human oral mucosa in health and oral chronic inflammatory disease. We deconvolved expression for resolution enhancement of spatial transcriptomic data and defined highly specialised epithelial and stromal compartments describing location-specific immune programs. Furthermore, we spatially mapped a rare pathogenic fibroblast population localised in a highly immunogenic region, responsible for lymphocyte recruitment through CXCL8 and CXCL10 and with a possible role in pathological angiogenesis through ALOX5AP. Collectively, our study provides a comprehensive reference for the study of oral chronic disease pathogenesis.Competing Interest StatementThe authors have declared that no conflict of interest exists. However, E. DA is an employee of Unilever Plc.