PT  - JOURNAL ARTICLE
AU  - Emerson Krock
AU  - Carlos E. Morado-Urbina
AU  - Joana Menezes
AU  - Matthew A. Hunt
AU  - Angelica Sandström
AU  - Diana Kadetoff
AU  - Jeanette Tour
AU  - Vivek Verma
AU  - Kim Kultima
AU  - Lisbet Haglund
AU  - Carolina B. Meloto
AU  - Luda Diatchenko
AU  - Eva Kosek
AU  - Camilla I. Svensson
TI  - Fibromyalgia patients with high levels of anti-satellite glia cell IgG antibodies present with more severe symptoms
AID  - 10.1101/2022.07.06.498940
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.07.06.498940
4099  - http://biorxiv.org/content/early/2022/07/06/2022.07.06.498940.short
4100  - http://biorxiv.org/content/early/2022/07/06/2022.07.06.498940.full
AB  - Objective Transferring fibromyalgia patient IgG to mice induces pain-like behaviour and fibromyalgia IgG binds mouse and human satellite glia cells (SGCs). These findings suggest that autoantibodies could be part of fibromyalgia pathology. However, it is unknown how frequently fibromyalgia patients have anti-SGC antibodies and how anti-SGC antibodies associate with disease severity.Methods We quantified serum or plasma anti-SGC IgG levels in two fibromyalgia cohorts from Sweden and Canada using an indirect immunofluorescence murine cell culture assay. Fibromyalgia serum IgG binding to human SGCs in human dorsal root ganglia tissue sections was assessed by immunofluorescence (n=14/group).Results In the cell culture assay anti-SGC IgG levels were increased in both fibromyalgia cohorts compared to controls. Elevated anti-SGC IgG was associated with higher levels of self-reported pain in both cohorts, and higher fibromyalgia impact questionnaire scores and increased pressure sensitivity in the Swedish cohort. Anti-SGC IgG levels were not associated with fibromyalgia duration. Swedish FM patients were clustered into FM-severe and FM-mild groups and the FM-severe group had elevated anti-SGC IgG compared to the FM-mild and controls. Anti-SGC IgG levels detected in culture were positively correlated with increased binding to human SGCs. Moreover, the FM-severe group had elevated IgG binding to human SGCs compared to the FM-mild and control groups.Conclusions A subset of fibromyalgia patients have elevated levels of anti-SGC antibodies, and the antibodies are associated with more severe fibromyalgia severity. Screening fibromyalgia patients for anti-SGC antibodies could provide a path to personalized treatment options that target autoantibodies and autoantibody production.Competing Interest StatementThe authors have declared no competing interest.