TY - JOUR T1 - Modular automated microfluidic cell culture platform reduces glycolytic stress in cerebral cortex organoids JF - bioRxiv DO - 10.1101/2022.07.13.499938 SP - 2022.07.13.499938 AU - Spencer T. Seiler AU - Gary L. Mantalas AU - John Selberg AU - Sergio Cordero AU - Sebastian Torres-Montoya AU - Pierre V. Baudin AU - Victoria T. Ly AU - Finn Amend AU - Liam Tran AU - Ryan N. Hoffman AU - Marco Rolandi AU - Richard E. Green AU - David Haussler AU - Sofie R. Salama AU - Mircea Teodorescu Y1 - 2022/01/01 UR - http://biorxiv.org/content/early/2022/07/14/2022.07.13.499938.abstract N2 - Organ-on-a-chip systems combine microfluidics, cell biology, and tissue engineering to culture 3D organ-specific in vitro models that recapitulate the biology and physiology of their in vivo counterparts. Here, we have developed a multiplex platform that automates the culture of individual organoids in isolated microenvironments at user-defined media flow rates. Programmable workflows allow the use of multiple reagent reservoirs that may be applied to direct differentiation, study temporal variables, and grow cultures long term. Novel techniques in polydimethylsiloxane (PDMS) chip fabrication are described here that enable features on the upper and lower planes of a single PDMS substrate. RNA sequencing (RNA-seq) analysis of automated cerebral cortex organoid cultures shows benefits in reducing glycolytic and endoplasmic reticulum stress compared to conventional in vitro cell cultures.Competing Interest StatementS.T.S and G.L.M. are founders of OrganOmics, a company that may be affected by the research reported in the enclosed paper. All other authors declare no competing interests. ER -