TY - JOUR T1 - mTORC2 couples fasting to mitochondrial fission JF - bioRxiv DO - 10.1101/2022.07.19.500669 SP - 2022.07.19.500669 AU - Nuria Martinez-Lopez AU - Pamela Mattar AU - Miriam Toledo AU - Henrietta Bains AU - Manu Kalyani AU - Marie Louise Aoun AU - Mridul Sharma AU - Laura Beth J. McIntyre AU - Leslie Gunther-Cummins AU - Frank P. Macaluso AU - Jennifer T. Aguilan AU - Simone Sidoli AU - Mathieu Bourdenx AU - Rajat Singh Y1 - 2022/01/01 UR - http://biorxiv.org/content/early/2022/07/20/2022.07.19.500669.abstract N2 - Fasting triggers diverse cellular and metabolic adaptations to facilitate organismal survival1,2. During nutrient deprivation, increases in circulating fatty acids support mitochondrial respiration2. The mechanisms driving mitochondrial adaptations and respiratory sufficiency during nutrient deprivation remain incompletely understood. Here we show that extended periods of fasting, or lipid availability stimulates mTORC2 activity. Activation of mTORC2 and phosphorylation of its target NDRG13 at S336 sustains mitochondrial fission and respiratory sufficiency. Timelapse imaging reveals that wildtype NDRG1, but not phosphorylation-deficient NDRG1S336A mutant, engages with mitochondria to facilitate its scission. Using proteomics, and an siRNA screen, we show that mTORC2-phosphorylated NDRG1 cooperates with the small GTPase Cdc424 and Cdc42-specific effectors and regulators to orchestrate fission. Accordingly, RictorKO, NDRG1S336A mutants, and Cdc42-deficient cells each display mitochondrial phenotypes reminiscent of fission failure. During nutrient surplus, mTOR complexes perform anabolic functions5; however, paradoxical reactivation of mTORC2 during fasting plays an unexpected role in driving mitochondrial fission and respiration.Competing Interest StatementThe authors have declared no competing interest. ER -