RT Journal Article
SR Electronic
T1 Lateral Line Ablation by Ototoxic Compounds Results in Distinct Rheotaxis Profiles in Larval Zebrafish
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.11.15.468723
DO 10.1101/2021.11.15.468723
A1 Kyle C Newton
A1 Dovi Kacev
A1 Simon R O Nilsson
A1 Allison L Saettele
A1 Sam A Golden
A1 Lavinia Sheets
YR 2022
UL http://biorxiv.org/content/early/2022/07/21/2021.11.15.468723.abstract
AB The zebrafish lateral line is an established model for hair cell organ damage, yet few studies link mechanistic disruptions to changes in biologically relevant behavior. We used larval zebrafish to determine how damage via ototoxic compounds impact rheotaxis. Larvae were treated with CuSO4 or neomycin to disrupt lateral line function then exposed to water flow stimuli. Their swimming behavior was recorded on video then DeepLabCut and SimBA software were used to track movements and classify rheotaxis behavior, respectively. Lateral line-disrupted fish performed rheotaxis, but they swam greater distances, for shorter durations, and with greater angular variance than controls. Furthermore, spectral decomposition analyses confirmed that lesioned fish exhibited ototoxic compound-specific behavioral profiles with distinct changes in the magnitude, frequency, and cross-correlation between fluctuations in linear and angular movements. Our observations demonstrate that lateral line input is needed for fish to hold their station in flow efficiently and reveals that commonly used lesion methods have unique effects on rheotaxis behavior.Competing Interest StatementThe authors have declared no competing interest.