TY - JOUR T1 - A self-blinking DNA probe for live-cell superresolution 3D imaging of hierarchical chromatin structures JF - bioRxiv DO - 10.1101/2022.06.22.497271 SP - 2022.06.22.497271 AU - Yang Zheng AU - Sen Ye AU - Shumin Li AU - Cuifang Liu AU - Shihang Luo AU - Yanqin Chen AU - Yunsheng Li AU - Lingyi Huang AU - Shan Deng AU - Ping Chen AU - Yongdeng Zhang AU - Wei Ji AU - Ruibang Luo AU - Guohong Li AU - Dan Yang Y1 - 2022/01/01 UR - http://biorxiv.org/content/early/2022/07/24/2022.06.22.497271.abstract N2 - How the 2-m-long genomic DNA is packaged into chromatin in the 10-µm eukaryotic nucleus is a fundamental question in cell biology. DNA accessibility depends on chromatin structure and dynamics, which basically control all DNA-related processes, such as transcription, DNA replication and repair. The hierarchical model of chromatin organization has been controversial in recent years because live-cell evidence for the existence of chromatin fibers, particularly those 30 nm in diameter, has remained elusive. Herein, we report a DNA-binding, fluorogenic and self-blinking small-molecule probe for the first live-cell 3D superresolution imaging of chromatin fibers with structural plasticity and fast dynamics. Selective inhibition of histone deacetylases led to decompaction of chromatin fibers due to hyperacetylation of histones. Our live-cell imaging results suggest a model of DNA packaging in interphase with the hierarchical organization of nucleosomal arrays and chromatin fibers.Competing Interest StatementThe authors have declared no competing interest. ER -