PT  - JOURNAL ARTICLE
AU  - Hu, Yucen
AU  - Xu, Jingxiu
AU  - Gao, Erqing
AU  - Fan, Xueyuan
AU  - Wei, Jieli
AU  - Xu, Suhong
AU  - Ma, Weirui
TI  - Enhanced Single RNA Imaging Reveals Dynamic Gene Expression in Live Animals
AID  - 10.1101/2022.07.26.501631
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.07.26.501631
4099  - http://biorxiv.org/content/early/2022/07/28/2022.07.26.501631.short
4100  - http://biorxiv.org/content/early/2022/07/28/2022.07.26.501631.full
AB  - Imaging endogenous mRNAs in live animals is technically challenging. Here we describe an MS2 based signal Amplification with Suntag System that enables live-cell RNA imaging of high temporal resolution and with 8xMS2 stem-loops, which overcomes the obstacle of inserting a 1,300 nt 24xMS2 into the genome for the imaging of endogenous mRNAs. Using this tool we were able to image the activation of gene expression and the dynamics of endogenous mRNAs in the epidermis of live C. elegans.Competing Interest StatementThe authors have declared no competing interest.