RT Journal Article
SR Electronic
T1 Enhanced Single RNA Imaging Reveals Dynamic Gene Expression in Live Animals
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.07.26.501631
DO 10.1101/2022.07.26.501631
A1 Hu, Yucen
A1 Xu, Jingxiu
A1 Gao, Erqing
A1 Fan, Xueyuan
A1 Wei, Jieli
A1 Xu, Suhong
A1 Ma, Weirui
YR 2022
UL http://biorxiv.org/content/early/2022/07/28/2022.07.26.501631.abstract
AB Imaging endogenous mRNAs in live animals is technically challenging. Here we describe an MS2 based signal Amplification with Suntag System that enables live-cell RNA imaging of high temporal resolution and with 8xMS2 stem-loops, which overcomes the obstacle of inserting a 1,300 nt 24xMS2 into the genome for the imaging of endogenous mRNAs. Using this tool we were able to image the activation of gene expression and the dynamics of endogenous mRNAs in the epidermis of live C. elegans.Competing Interest StatementThe authors have declared no competing interest.