PT  - JOURNAL ARTICLE
AU  - Enric Llorens-Bobadilla
AU  - Margherita Zamboni
AU  - Maja Marklund
AU  - Nayanika Bhalla
AU  - Xinsong Chen
AU  - Johan Hartman
AU  - Jonas Frisén
AU  - Patrik L Ståhl
TI  - Chromatin accessibility profiling in tissue sections by spatial ATAC
AID  - 10.1101/2022.07.27.500203
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.07.27.500203
4099  - http://biorxiv.org/content/early/2022/07/29/2022.07.27.500203.short
4100  - http://biorxiv.org/content/early/2022/07/29/2022.07.27.500203.full
AB  - Current methods for epigenomic profiling are limited in the ability to obtain genome wide information with spatial resolution. Here we introduce spatial ATAC, a method that integrates transposase-accessible chromatin profiling in tissue sections with barcoded solid-phase capture to perform spatially resolved epigenomics. We show that spatial ATAC enables the discovery of the regulatory programs underlying spatial gene expression during mouse organogenesis, lineage differentiation and in human pathological samples.Competing Interest StatementE.L.B., M.Z., M.M., N.B., J.F. and P.L.S. are scientific consultants to 10x Genomics, which holds IP rights to the spatial technology.