RT Journal Article
SR Electronic
T1 TraceTrack, an Open-Source Software for Batch Processing, Alignment and Visualization of Sanger Sequencing Chromatograms
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.07.28.501824
DO 10.1101/2022.07.28.501824
A1 Brazdilova, Kveta
A1 Prihoda, David
A1 Ton, Quynh
A1 Klock, Heath
A1 Bitton, Danny A.
YR 2022
UL http://biorxiv.org/content/early/2022/08/01/2022.07.28.501824.abstract
AB Background Despite the advent of Next Generation Sequencing technology and its widespread applications, Sanger sequencing remains instrumental for molecular biology subcloning work in biological and medical research and indispensable for drug discovery campaigns. Although Sanger sequencing technology has been long established, existing software for processing and visualization of trace file chromatograms are limited in terms of functionality, scalability, and availability for commercial use.Results To fill this gap, we developed TraceTrack, an open-source web application tool for batch alignment, analysis and visualization of Sanger trace files. TraceTrack offers high throughput matching of trace files to reference sequences, rapid identification of mutations and an intuitive chromatogram analysis. Comparative analysis between TraceTrack and existing software tools highlights the advantages of TraceTrack with regards to batch processing, visualization and export functionalities. TraceTrack is available at https://github.com/Merck/TraceTrack and also at https://tracetrack.dichlab.org as a web application.Conclusion TraceTrack is a web application for batch processing and visualization of Sanger trace file chromatograms that meets the increasing demand of industrial sequence validation workflows in pharmaceutical settings.Competing Interest StatementAll authors that are/were employees of Merck Sharp & Dohme LLC, a subsidiary of Merck & Co., Inc., Rahway, NJ, USA and may hold stocks and/or stock options in Merck & Co., Inc., Rahway, NJ, USA.MSAMultiple sequence alignmentNGSNext generation sequencingSTRShort tandem repeat