RT Journal Article
SR Electronic
T1 DNA Origami Presenting the Receptor Binding Domain of SARS-CoV-2 Elicit Robust Protective Immune Response
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.08.02.502186
DO 10.1101/2022.08.02.502186
A1 Esra Oktay
A1 Farhang Alem
A1 Keziah Hernandez
A1 Aarthi Narayanan
A1 Remi Veneziano
YR 2022
UL http://biorxiv.org/content/early/2022/08/02/2022.08.02.502186.abstract
AB Effective and safe vaccines are invaluable tools in the arsenal to fight infectious diseases. The rapid spreading of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) responsible of the coronavirus disease 2019 pandemic has highlighted the need to develop methods for rapid and efficient vaccine development. DNA origami nanoparticles (DNA-NPs) presenting multiple antigens in prescribed nanoscale patterns have recently emerged as a safe, efficient, and easily scalable alternative for rational design of vaccines. Here, we are leveraging the unique properties of these DNA-NPs and demonstrate that precisely patterning ten copies of a reconstituted trimer of the receptor binding domain (RBD) of SARS-CoV-2 along with CpG adjuvants on the DNA-NPs is able to elicit a robust protective immunity against SARS-CoV-2 in a mouse model. Our results demonstrate the potential of our DNA-NP-based approach for developing safe and effective nanovaccines against infectious diseases.Competing Interest StatementDr. Veneziano is listed as an inventor on submitted patents related to this work. All other authors declare no conflict of interests.