@article {Gallaher2022.08.04.502852, author = {Jill Gallaher and Maximilian Strobl and Jeffrey West and Jingsong Zhang and Robert Gatenby and Mark Robertson-Tessi and Alexander R. A. Anderson}, title = {The sum and the parts: dynamics of multiple and individual metastases during adaptive therapy}, elocation-id = {2022.08.04.502852}, year = {2022}, doi = {10.1101/2022.08.04.502852}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Evolutionary therapies, such as adaptive therapy, have shown promise in delaying treatment resistance in late-stage cancers. By alternating between drug applications and drug-free vacations, competition between sensitive and resistant cells can be exploited to maximize the time to progression. However, the optimal schedule of this dosing regimen depends on the properties of the tumor, which often are not directly measurable in clinical practice. In this work, we propose that the initial cycle of adaptive therapy can be used as a tool to probe the relevant tumor properties. We present a framework for estimating individual and collective components of a metastatic system through tumor response dynamics, which uses a system of off-lattice agent-based models to represent individual metastatic lesions within independent domains, all of which are subject to the same systemic therapy. We find that the first cycle of adaptive therapy delineates several features of the computational metastatic system: larger metastases have longer cycles; a higher proportion of drug resistant cells slows the cycles; and a faster cell turnover rate speeds up drug response time and slows the regrowth time. The number of metastases does not affect cycle times, as response dynamics are dominated by the largest tumors rather than the aggregate. In addition, the heterogeneity of the system is also a guide for therapeutic approaches: generally, systems with more between-tumor (intertumor) heterogeneity had better success with continuous therapy, while systems with more within-tumor (intratumor) heterogeneity responded better to adaptive therapy. Intertumor heterogeneity was found to correlate more with dynamics from patients with high and low Gleason scores while intratumor heterogeneity was correlated with dynamics from patients with intermediate Gleason scores.Competing Interest StatementThe authors have declared no competing interest.}, URL = {https://www.biorxiv.org/content/early/2022/08/04/2022.08.04.502852}, eprint = {https://www.biorxiv.org/content/early/2022/08/04/2022.08.04.502852.full.pdf}, journal = {bioRxiv} }