RT Journal Article
SR Electronic
T1 Influenza A virus liquid condensates can undergo pharmacological hardening
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.08.03.502602
DO 10.1101/2022.08.03.502602
A1 Temitope Akhigbe Etibor
A1 Sindhuja Sridharan
A1 Sílvia Vale-Costa
A1 Daniela Brás
A1 Isabelle Becher
A1 Victor Mello
A1 Filipe Ferreira
A1 Marta Alenquer
A1 Mikhail M Savitski
A1 Maria João Amorim
YR 2022
UL http://biorxiv.org/content/early/2022/08/05/2022.08.03.502602.abstract
AB Multiple viral infections form biomolecular condensates in the host cell to facilitate viral replication1. Accumulating evidence indicates that these viral condensates rely on specific material properties for function 2, but how these properties may be altered efficiently remains vastly unknown. Here, we use influenza A virus liquid cytosolic condensates 3, A.K.A viral inclusions, to provide a proof of concept that stabilizing transient interactions among the interactome in IAV inclusions more efficiently hardens these structures than varying temperature or concentration both in in situ and in in vivo models. This stabilization can be achieved by drug targeting, inducing changes in the solubility of viral proteome without affecting host cellular proteome. Our work supports the development of antivirals targeting the material properties of biomolecular condensates in viral infections. It also provides a framework for the efficient selection of pharmacological compounds with this activity and thus provides an advance in disease therapy.Competing Interest StatementThe authors have declared no competing interest.