TY - JOUR T1 - Neuronal SNAP-23 scales hippocampal synaptic plasticity and memory JF - bioRxiv DO - 10.1101/2022.08.04.502541 SP - 2022.08.04.502541 AU - Mengjia Huang AU - Na-Ryum Bin AU - Jayant Rai AU - Ke Ma AU - Chun Hin Chow AU - Sarah Eide AU - Hidekiyo Harada AU - Jianbing Xiao AU - Daorong Feng AU - Hong-Shuo Sun AU - Zhong-Ping Feng AU - Herbert Y. Gaisano AU - Jeffrey E. Pessin AU - Philippe P. Monnier AU - Kenichi Okamoto AU - Liang Zhang AU - Shuzo Sugita Y1 - 2022/01/01 UR - http://biorxiv.org/content/early/2022/08/06/2022.08.04.502541.abstract N2 - Soluble NSF Attachment protein REceptor (SNARE)-mediated membrane fusion plays a crucial role not only in presynaptic vesicle exocytosis but also in postsynaptic receptor delivery. The latter is considered particularly important for long-term synaptic plasticity and learning and memory, yet underlying mechanisms including the identity of the key SNARE proteins remain elusive. Here, we investigate the role of neuronal Synaptosomal-Associated Protein-23 (SNAP-23) by analyzing pyramidal-neuron specific SNAP-23 conditional knockout (cKO) mice. SNAP-23 immunostaining in postsynaptic spines was effectively decreased in the SNAP-23 cKO hippocampus. Electrophysiological analysis of SNAP-23 deficient neurons using acute hippocampal slices showed normal basal neurotransmission in CA3-CA1 synapses with unchanged AMPA and NMDA currents. Nevertheless, we found theta-burst stimulation induced long-term potentiation (LTP) was vastly diminished in SNAP-23 cKO. Moreover, unlike syntaxin-4 cKO mice in which both basal neurotransmission and LTP decrease manifested changes in a broad set of behavioral tasks, deficits of SNAP-23 cKO is more limited to spatial memory. Our data reveal that neuronal SNAP-23 is selectively crucial for synaptic plasticity and spatial memory without affecting basal glutamate receptor function.Competing Interest StatementThe authors have declared no competing interest. ER -