PT  - JOURNAL ARTICLE
AU  - Nicholas I. Clarke
AU  - Stephen J. Royle
TI  - FerriTag: A Genetically-Encoded Inducible Tag for Correlative Light-Electron Microscopy
AID  - 10.1101/095208
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 095208
4099  - http://biorxiv.org/content/early/2016/12/18/095208.short
4100  - http://biorxiv.org/content/early/2016/12/18/095208.full
AB  - A current challenge is to develop tags to precisely visualize proteins in cells by light and electron microscopy. Here, we introduce FerriTag, a genetically-encoded chemically-inducible tag for correlative light-electron microscopy (CLEM). FerriTag is a fluorescent recombinant electron-dense ferritin particle that can be attached to a protein-of-interest using rapamycin-induced heterodimerization. We demonstrate the utility of FerriTag for CLEM by labeling proteins associated with various intracellular structures including mitochondria, plasma membrane, and clathrin-coated pits and vesicles. FerriTagging has a high signal-to-noise ratio and a labeling resolution of 10 ± 5 nm. We demonstrate how FerriTagging allows nanoscale mapping of protein location relative to a subcellular structure, and use it to detail the distribution of huntingtin-interacting protein 1 related (HIP1R) in clathrin-coated pits.