RT Journal Article
SR Electronic
T1 Distinct mechanisms underlie H<sub>2</sub>O<sub>2</sub> sensing in <em>C. elegans</em> head and tail
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.07.26.451501
DO 10.1101/2021.07.26.451501
A1 Sophie Quintin
A1 Théo Aspert
A1 Tao Ye
A1 Gilles Charvin
YR 2022
UL http://biorxiv.org/content/early/2022/08/11/2021.07.26.451501.abstract
AB Environmental oxidative stress threatens cellular integrity and should therefore be avoided by living organisms. Yet, relatively little is known about environmental oxidative stress perception. Here, using microfluidics, we showed that like I2 pharyngeal neurons, the tail phasmid PHA neurons function as oxidative stress sensing neurons in C. elegans, but display different responses to H2O2 and light. We uncovered that different but related receptors, GUR-3 and LITE-1, mediate H2O2 signaling in I2 and PHA neurons. Still, the peroxiredoxin PRDX-2 is essential for both, and might promote H2O2-mediated receptor activation. Our work demonstrates that C. elegans can sense a broad range of oxidative stressors using partially distinct H2O2 signaling pathways in head and tail sensillae, and paves the way for further understanding of how the integration of these inputs translates into the appropriate behavior.Competing Interest StatementThe authors have declared no competing interest.