PT  - JOURNAL ARTICLE
AU  - Sekar, Vaishnovi
AU  - Mármol-Sánchez, Emilio
AU  - Kalogeropoulos, Panagiotis
AU  - Stanicek, Laura
AU  - Sagredo, Eduardo A.
AU  - Doukoumopoulos, Evangelos
AU  - Bonath, Franziska
AU  - Biryukova, Inna
AU  - Friedländer, Marc R.
TI  - agoTRIBE detects miRNA-target interactions transcriptome-wide in single cells
AID  - 10.1101/2022.08.10.503472
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.08.10.503472
4099  - http://biorxiv.org/content/early/2022/08/11/2022.08.10.503472.short
4100  - http://biorxiv.org/content/early/2022/08/11/2022.08.10.503472.full
AB  - MicroRNAs are gene regulatory molecules that play important roles in numerous biological processes including human health. The function of a given microRNA is defined by its selection of target transcripts, yet current state-of-the-art experimental methods to identify microRNA targets are laborious and require millions of cells. We have overcome these limitations by fusing the microRNA effector protein Argonaute2 to the RNA editing domain of ADAR2, allowing for the first time the detection of microRNA targets transcriptome-wide in single cells. Our agoTRIBE method reports functional microRNA targets which are additionally supported by evolutionary sequence conservation. As a proof-of-principle, we study microRNA interactions in single cells, and find substantial differential targeting across the cell cycle. Lastly, agoTRIBE additionally provides transcriptome-wide measurements of RNA abundance and will allow the deconvolution of microRNA targeting in complex samples such as tissues at the single-cell level.Competing Interest StatementThe authors have declared no competing interest.