RT Journal Article
SR Electronic
T1 agoTRIBE detects miRNA-target interactions transcriptome-wide in single cells
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.08.10.503472
DO 10.1101/2022.08.10.503472
A1 Vaishnovi Sekar
A1 Emilio Mármol-Sánchez
A1 Panagiotis Kalogeropoulos
A1 Laura Stanicek
A1 Eduardo A. Sagredo
A1 Evangelos Doukoumopoulos
A1 Franziska Bonath
A1 Inna Biryukova
A1 Marc R. Friedländer
YR 2022
UL http://biorxiv.org/content/early/2022/08/11/2022.08.10.503472.abstract
AB MicroRNAs are gene regulatory molecules that play important roles in numerous biological processes including human health. The function of a given microRNA is defined by its selection of target transcripts, yet current state-of-the-art experimental methods to identify microRNA targets are laborious and require millions of cells. We have overcome these limitations by fusing the microRNA effector protein Argonaute2 to the RNA editing domain of ADAR2, allowing for the first time the detection of microRNA targets transcriptome-wide in single cells. Our agoTRIBE method reports functional microRNA targets which are additionally supported by evolutionary sequence conservation. As a proof-of-principle, we study microRNA interactions in single cells, and find substantial differential targeting across the cell cycle. Lastly, agoTRIBE additionally provides transcriptome-wide measurements of RNA abundance and will allow the deconvolution of microRNA targeting in complex samples such as tissues at the single-cell level.Competing Interest StatementThe authors have declared no competing interest.