RT Journal Article SR Electronic T1 A low-cost, open-source evolutionary bioreactor and its educational use JF bioRxiv FD Cold Spring Harbor Laboratory SP 729434 DO 10.1101/729434 A1 Vishhvaan Gopalakrishnan A1 Dena Crozier A1 Kyle J. Card A1 Lacy D. Chick A1 Nikhil P. Krishnan A1 Erin McClure A1 Julia Pelesko A1 Drew F.K. Williamson A1 Daniel Nichol A1 Soumyajit Mandal A1 Robert A. Bonomo A1 Jacob G. Scott YR 2022 UL http://biorxiv.org/content/early/2022/09/01/729434.1.abstract AB The morbidostat automatically adjusts antibiotic concentration as a bacterial population evolves resistance. Although this device has advanced our understanding of the evolutionary and ecological processes that drive antibiotic resistance, no low-cost and open-source systems are available for educators. Here, we present the EVolutionary biorEactor (EVE), an accessible alternative to other morbidostats for use in low-resource classrooms that requires minimal engineering and programming experience. We first compare our system to others, emphasizing how it differs in design and cost. We then describe how we validated the EVE by evolving replicate Escherichia coli populations under chloramphenicol challenge and comparing our results to those in the published literature. Lastly, we detail how high school students used the EVE to learn about bacterial growth and antibiotic resistance.Competing Interest StatementThe authors have declared no competing interest.