@article {Long096388, author = {Xi Long and Jennifer Colonell and Allan M Wong and Robert H Singer and Timoth{\'e}e Lionnet}, title = {Quantitative mRNA Imaging Throughout the Entire Drosophila Brain}, elocation-id = {096388}, year = {2016}, doi = {10.1101/096388}, publisher = {Cold Spring Harbor Laboratory}, abstract = {We describe a fluorescence in situ hybridization method that permits detection of the localization and abundance of single mRNAs (smFISH) in cleared whole-mount adult Drosophila brains. The approach is rapid, multiplexable and does not require molecular amplification; it allows facile mRNA expression quantification with subcellular resolution on a standard confocal microscope. Using a custom Bessel Beam-Structured Illumination microscope (BB-SIM), we further demonstrate single-mRNA detection across the entire brain sample.}, URL = {https://www.biorxiv.org/content/early/2016/12/22/096388}, eprint = {https://www.biorxiv.org/content/early/2016/12/22/096388.full.pdf}, journal = {bioRxiv} }