RT Journal Article
SR Electronic
T1 Molecular characterization of the intact muscle spindle using a multi-omics approach
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.07.13.499888
DO 10.1101/2022.07.13.499888
A1 Bavat Bornstein
A1 Lia Heinemann-Yerushalmi
A1 Sharon Krief
A1 Ruth Adler
A1 Bareket Dassa
A1 Dena Leshkowitz
A1 Minchul Kim
A1 Guy Bewick
A1 Robert W. Banks
A1 Elazar Zelzer
YR 2022
UL http://biorxiv.org/content/early/2022/09/06/2022.07.13.499888.abstract
AB The proprioceptive system is essential for the control of coordinated movement, posture and skeletal integrity. The sense of proprioception is produced in the brain using peripheral sensory input from receptors such as the muscle spindle, which detects changes in the length of skeletal muscles. Despite its importance, the molecular composition of the muscle spindle is largely unknown. In this study, we generated comprehensive transcriptomic and proteomic datasets of the entire muscle spindle. We then associated differentially expressed genes with the various tissues composing the spindle using bioinformatic analysis. Immunostaining verified these predictions, thus establishing new markers for the different spindle tissues. Utilizing these markers, we identified the differentiation stages the spindle capsule cells undergo during development. Together, these findings provide comprehensive molecular characterization of the intact spindle as well as new tools to study its development and function in health and disease.Competing Interest StatementThe authors have declared no competing interest.