RT Journal Article
SR Electronic
T1 Genetic and transgenic reagents for <em>Drosophila simulans, D. mauritiana, D. yakuba, D. santomea</em> and <em>D. virilis</em>
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 096644
DO 10.1101/096644
A1 David L. Stern
A1 Justin Crocker
A1 Yun Ding
A1 Nicolas Frankel
A1 Gretchen Kappes
A1 Elizabeth Kim
A1 Ryan Kuzmickas
A1 Andrew Lemire
A1 Serge Picard
YR 2016
UL http://biorxiv.org/content/early/2016/12/23/096644.abstract
AB Species of the Drosophila melanogaster species subgroup, including the species D. simulans, D. mauritiana, D. yakuba, and D. santomea, have long served as model systems for studying evolution. Studies in these species have been limited, however, by a paucity of genetic and transgenic reagents. Here we describe a collection of transgenic and genetic strains generated to facilitate genetic studies within and between these species. We have generated many strains of each species containing mapped piggyBac transposons including an enhanced yellow fluorescent protein gene expressed in the eyes and a phiC31 attP site-specific integration site. We have tested a subset of these lines for integration efficiency and reporter gene expression levels. We have also generated a smaller collection of other lines expressing other genetically encoded fluorescent molecules in the eyes and a number of other transgenic reagents that will be useful for functional studies in these species. In addition, we have mapped the insertion locations of 86 transposable elements in D. virilis that will be useful for genetic mapping studies.