RT Journal Article SR Electronic T1 Natural variation in codon bias and mRNA folding strength interact synergistically to modify protein expression in Saccharomyces cerevisiae JF bioRxiv FD Cold Spring Harbor Laboratory SP 2022.09.07.507001 DO 10.1101/2022.09.07.507001 A1 Anastacia N. Wienecke A1 Margaret L. Barry A1 Daniel A. Pollard YR 2022 UL http://biorxiv.org/content/early/2022/09/09/2022.09.07.507001.abstract AB Codon bias and mRNA folding strength (mF) are hypothesized molecular mechanisms by which polymorphisms in genes modify protein expression. Natural patterns of codon bias and mF across genes as well as effects of altering codon bias and mF suggest the influence of these two mechanisms may vary depending on the specific location of polymorphisms within a transcript. Despite the central role codon bias and mF may play in natural trait variation within populations, systematic studies of how polymorphic codon bias and mF relate to protein expression variation are lacking. To address this need, we analyzed genomic, transcriptomic, and proteomic data for 22 Saccharomyces cerevisiae isolates, estimated protein accumulation for each allele of 1620 genes as the log of protein molecules per RNA molecule (logPPR), and built linear mixed effects models associating allelic variation in codon bias and mF with allelic variation in logPPR. We found codon bias and mF interact synergistically in a positive association with logPPR and this interaction explains almost all the effect of codon bias and mF. We examined how the locations of polymorphisms within transcripts influence their effects and found that codon bias primarily acts through polymorphisms in domain encoding and 3’ coding sequences while mF acts most significantly through coding sequences with weaker effects from UTRs. Our results present the most comprehensive characterization to date of how polymorphisms in transcripts influence protein expression.