TY - JOUR T1 - Quantification of membrane binding and diffusion using Fluorescence Correlation Spectroscopy diffusion laws JF - bioRxiv DO - 10.1101/2022.09.12.507540 SP - 2022.09.12.507540 AU - Anita Mouttou AU - Erwan Bremaud AU - Julien Noero AU - Rayane Dibsy AU - Coline Arone AU - Johnson Mak AU - Delphine Muriaux AU - Hugues Berry AU - Cyril Favard Y1 - 2022/01/01 UR - http://biorxiv.org/content/early/2022/09/14/2022.09.12.507540.abstract N2 - Many transient processes in cells arise from the binding of cytosolic proteins to membranes. Quantifying this membrane binding and its associated diffusion in the living cell is therefore of primary importance. Dynamic photonic microscopies, e.g. single/multiple particle tracking, fluorescence recovery after photobleaching and fluorescence correlation spectroscopy (FCS) enable noninvasive measurement of molecular mobility in living cells and their plasma membranes. However, FCS with a single beam waist is of limited applicability with complex, non Brownian, motions. Recently, the development of FCS diffusion laws methods has given access to the characterization of these complex motions, although none of them is applicable to the membrane binding case at the moment. In this study, we combined computer simulations and FCS experiments to propose an FCS diffusion law for membrane binding. First, we generated computer simulations of spot-variation FCS (svFCS) measurements for a membrane binding process combined to 2D and 3D diffusion at the membrane and in the bulk/cytosol, respectively. Then, using these simulations as a learning set, we derived an empirical diffusion law with three free parameters: the apparent binding constant KDapp, the diffusion coefficient on the membrane D2D and the diffusion coefficient in the bulk/cytosol, D3D. Finally, we monitored, using svFCS, the dynamics of retroviral Gag proteins and associated mutants during their binding to supported lipid bilayers of different lipid composition or at plasma membranes of living cells and we quantified KDapp and D2D in these conditions using our empirical diffusion law. Based on these experiments and numerical simulations, we conclude that this new approach enables correct estimation of membrane partitioning and membrane diffusion properties (KDapp and D2D) for peripheral membrane molecules.Competing Interest StatementThe authors have declared no competing interest. ER -