PT  - JOURNAL ARTICLE
AU  - Mejia, Catalina
AU  - Trujillo Rodriguez, Lidimarie
AU  - Poudel, Ravin
AU  - Ellington, Adam
AU  - Rivers, Adam R.
AU  - Reisch, Christopher R.
TI  - An Arrayed Transposon Library of <em>Ruegeria pomeroyi</em> DSS-3
AID  - 10.1101/2022.09.11.507510
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.09.11.507510
4099  - http://biorxiv.org/content/early/2022/09/14/2022.09.11.507510.short
4100  - http://biorxiv.org/content/early/2022/09/14/2022.09.11.507510.full
AB  - The ability to construct defined genetic mutations in many bacteria is difficult and limited. Transposon mutagenesis is often highly efficient, but is not site specific, thus selections are often needed to identify mutants of interest. The construction of arrayed mutant libraries would help to fill this need, though these libraries are costly and time consuming. To enable easier construction of arrayed libraries we developed a workflow and methodology using a hierarchical barcoding scheme to identify mutants within a multiwell plate. We applied this method to the marine Alphaproteobacterium Ruegeria pomeroyi DSS-3 and created a library with over 2,800 disrupted genes.Competing Interest StatementThe authors have declared no competing interest.